About Master of Pores

Master of Pores is a pipeline writte in Nextflow DSL2 for the analysis of Nanopore data. It can handle reads from direct RNAseq, cDNAseq, DNAseq etc.

The pipeline is composed by four modules:

  • mop_preprocess: preprocessing

  • mop_mod: detecting chemical modifications. It reads the output directly from mop_preprocess

  • mop_tail: estimating polyA tail size. It reads the output directly from mop_preprocess

  • mop_consensus: it generates a consensus from the predictions from mop_mod. It reads the output directly from mop_mod

The name is inspired by Metallica’s Master Of Puppets

_images/master_red.jpg

This is a joint project between CRG bioinformatics core and Epitranscriptomics and RNA Dynamics research group.

Reference

If you use this tool, please cite our paper:

“MasterOfPores: A Workflow for the Analysis of Oxford Nanopore Direct RNA Sequencing Datasets” Luca Cozzuto, Huanle Liu, Leszek P. Pryszcz, Toni Hermoso Pulido, Anna Delgado-Tejedor, Julia Ponomarenko, Eva Maria Novoa. Front. Genet., 17 March 2020.