Welcome to the documentation of Master Of Pores 2

Master of Pores is a pipeline written in Nextflow DSL2 for the analysis of Nanopore data. It can handle reads from direct RNAseq, cDNAseq, DNAseq etc.

The pipeline is composed by theee modules:

• mop_preprocess: preprocessing

• mop_mod: detecting chemical modifications. It reads the output directly from mop_preprocess.

• mop_tail: estimating polyA tail size. It reads the output directly from mop_preprocess

• mop_consensus: it generates a consensus from the predictions from mop_mod. It reads the output directly from mop_mod

Contents:

• About Master of Pores
• How to install
• MOP_PREPROCESS
• MOP_TAIL
• MOP_MOD
• MOP_CONSENSUS
• Resource reporting
• Benchmark
• CHANGELOG