About Master of Pores 4

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Master of Pores 4 is a collection of pipelines written in Nextflow DSL2 for the analysis of Nanopore data. It can handle reads from direct RNAseq, cDNAseq, DNAseq etc.

Master of Pores is a pipeline written in Nextflow DSL2 for the analysis of Nanopore data. It can handle reads from direct RNAseq, cDNAseq, DNAseq etc.

The package is currently composed of one pipeline, more will be soon released:

  • mop_preprocess: preprocessing of raw data

The name is inspired by Metallica’s Master Of Puppets

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This is a joint project between CRG bioinformatics core and Epitranscriptomics and RNA Dynamics research group.

Reference

If you use this tool, please cite our papers:

“Nanopore Direct RNA Sequencing Data Processing and Analysis Using MasterOfPores” Cozzuto L, Delgado-Tejedor A, Hermoso Pulido T, Novoa EM, Ponomarenko J. N. Methods Mol Biol. 2023;2624:185-205. doi: 10.1007/978-1-0716-2962-8_13.

“MasterOfPores: A Workflow for the Analysis of Oxford Nanopore Direct RNA Sequencing Datasets” Luca Cozzuto, Huanle Liu, Leszek P. Pryszcz, Toni Hermoso Pulido, Anna Delgado-Tejedor, Julia Ponomarenko, Eva Maria Novoa. Front. Genet., 17 March 2020.