logo RNAseq course 2019

Dates, time & location


Day Date Time
1 Monday 13th of May 2019 9:30-13:30
2 Tuesday 14th of May 2019 9:30-13:30
3 Wednesday 15th of May 2019 14:00-17:00
4 Thursday 16th of May 2019 14:00-17:00
5 Monday 27th of May 2019 9:30-13:00

Location: Carrer del Dr. Aiguader, 88, 08003 Barcelona. CRG Training center, PRBB building ground floor.


Luca Cozzuto Julia Ponomarenko Sarah Bonnin

from the CRG Bioinformatics core facility (office 460, 4th floor hotel side)

Material available at https://biocorecrg.github.io/RNAseq_course_2019/

Learning objectives

At the end of the course, the participants will be able to:

  • Understand the steps from raw reads to expression counts, differential expression and interpretation of gene lists using enrichment analysis
  • Define a good experimental design, including experimental design, sequencing design, and quality control steps)
  • Perform quality assessment of RNA-seq data, raw and processed
  • Understand file formats commonly used in RNA-seq data analysis
  • Gain an overview on common software tools for RNA-seq data analysis and their limitations
  • Run RNA-seq pipeline to perform differential expression analysis

Course Program

  • Day 1: Introduction, download raw data, quality control of data
  • Day 2: Read mapping to reference genome
  • Day 3: Differential expression analysis
  • Day 4: Genome browser, gene ontology enrichment analysis
  • Day 5: Troubleshooting of a mini-project


Basic experience of command line Linux