RNAseq course 2019
Dates, time & location
Dates:
| Day | Date | Time |
|---|---|---|
| 1 | Monday 13th of May 2019 | 9:30-13:30 |
| 2 | Tuesday 14th of May 2019 | 9:30-13:30 |
| 3 | Wednesday 15th of May 2019 | 14:00-17:00 |
| 4 | Thursday 16th of May 2019 | 14:00-17:00 |
| 5 | Monday 27th of May 2019 | 9:30-13:00 |
Location: Carrer del Dr. Aiguader, 88, 08003 Barcelona. CRG Training center, PRBB building ground floor.
Instructors
| Luca Cozzuto | Julia Ponomarenko | Sarah Bonnin |
|---|---|---|
 |
 |
 |
from the CRG Bioinformatics core facility (office 460, 4th floor hotel side)
Material available at https://biocorecrg.github.io/RNAseq_course_2019/
Learning objectives
At the end of the course, the participants will be able to:
- Understand the steps from raw reads to expression counts, differential expression and interpretation of gene lists using enrichment analysis
- Define a good experimental design, including experimental design, sequencing design, and quality control steps)
- Perform quality assessment of RNA-seq data, raw and processed
- Understand file formats commonly used in RNA-seq data analysis
- Gain an overview on common software tools for RNA-seq data analysis and their limitations
- Run RNA-seq pipeline to perform differential expression analysis
Course Program
- Day 1: Introduction, download raw data, quality control of data
- Day 2: Read mapping to reference genome
- Day 3: Differential expression analysis
- Day 4: Genome browser, gene ontology enrichment analysis
- Day 5: Troubleshooting of a mini-project