Read mapping to a reference genome/transcriptome
After sequencing reads are pre-processed and their quality is checked, next step is to map them to a reference genome or transcriptome.
|Mapping of short reads|
Tools for read mapping
Multiples aligners were developped over the last decades, using different algorithms:
|Read mappers timeline|
Before doing the mapping, we have to prepare an index from the reference DNA sequence that a chosen algorithm will use.
Like the index at the end of a book, an index of a large DNA sequence allows one to rapidly find shorter sequences embedded in it. Different tools use different approaches at genome/transcriptome indexing.
Fast (splice-unaware) aligners to a reference transcriptome
These tools can be used for aligning short reads to a transcriptome reference.
If a genome were used as a reference, these tools would not map reads to splicing junctions.
They can be much faster than traditional aligners like Blast but less sensitive and may have limitations about the read size.
- Bowtie is an ultrafast, memory-efficient short read aligner geared toward quickly aligning large sets of short DNA sequences (reads) to large genomes/transcriptomes. Bowtie uses a Burrows-Wheeler index.
- Bowtie2 is an ultrafast and memory-efficient tool for aligning sequencing reads to long reference sequences. It is particularly good at aligning reads of length 50 up to 100s or 1,000s to relatively long (e.g. mammalian) genomes. Bowtie 2 indexes the transcriptome with an FM Index.
- BWA is a software package for mapping low-divergent sequences against a large reference genome, such as the human genome. BWA indexes the genome with an FM Index.
- GEM is a high-performance mapping tool for aligning sequenced reads against large reference genomes. It is designed to obtain best results when mapping sequences up to 1K bases long. GEM3 indexes the reference genome using a custom FM-Index design and performs an adaptive gapped search based on the characteristics of the input and the user settings.
Splice-aware aligners to a reference genome
These aligners are able to map to the splicing junctions described in the annotation and even to detect novel ones.
Some of them can detect gene fusions and SNPs and also RNA editing. For some of these tools, the downstream analysis requires the assignation of the aligned reads to a given gene/transcript.
- Tophat is a fast splice junction mapper for RNA-Seq reads. It aligns RNA-Seq reads to mammalian-sized genomes using the ultra high-throughput short read aligner Bowtie, and then analyzes the mapping results to identify splice junctions between exons.
- HISAT2 is the next generation of spliced aligner from the same group that have developed TopHat. It is a fast and sensitive alignment program for mapping next-generation sequencing reads (both DNA and RNA) to a population of human genomes (as well as to a single reference genome). The indexing scheme is called a Hierarchical Graph FM index (HGFM).
- STAR is an ultrafast universal RNA-seq aligner. It uses sequential maximum mappable seed search in uncompressed suffix arrays followed by seed clustering and stitching procedure. It is also able to search for gene fusions.
Quasi-mappers (alignment-free mappers) to a reference transcriptome
These tools are way faster than the previous ones because they don’t need to report the resulting alignments (BAM/SAM files) but only associate a read to a given transcript for quantification. They don’t discover novel transcript variants (or splicing events) or detect variations, etc.
- Sailfish replaces read mapping with intelligent k-mer indexing and counting, thus allowing fast quantification of isoform abundance (the authors claim that it takes about 15 minutes for a set of 150 million reads).
- Salmon is an advanced version of Sailfish, by the same authors, tool for wicked-fast transcript quantification from RNA-seq data. It requires a set of target transcripts to quantify and a K-mer parameter to make the index (i.e. minimum acceptable alignment).
- Kallisto is a program for quantifying abundances of transcripts from bulk and single-cell RNA-Seq data. It is based on the novel idea of pseudoalignment for rapidly determining the compatibility of reads with targets, without the need for alignment.
For the results of different mappers/aligners comparison, see
Reference genome: FASTA and GTF/GFF
Before proceeding, we need to retrieve a reference genome or transcriptome from a public database, along with its annotation:
- A FASTA file contains the actual genome/transcriptome sequence.
- A GTF/GFF file contains the corresponding annotation.
Public resources on genome/transcriptome sequences and annotations
- GENCODE contains an accurate annotation of the human and mouse genes derived either using manual curation, computational analysis or targeted experimental approaches. GENCODE also contains information on functional elements, such as protein-coding loci with alternatively splices variants, non-coding loci and pseudogenes.
- Ensembl contains both automatically generated and manually curated annotations. They host different genomes and also comparative genomics data and variants. Ensembl genomes extends the genomic information across different taxonomic groups: bacteria, fungi, metazoa, plants, protists. Ensembl integrates also a genome browser.
- UCSC Genome Browser hosts information about different genomes. It integrates the GENCODE and Ensembl information as additional tracks.
Where to find the files
The current version for Homo sapiens genome is release 33.
The files you would need are:
- FASTA file for the Genome sequence, primary assembly
- FASTA file corresponding to the transcripts
- GTF file of the Comprehensive gene annotation
# genome wget ftp://ftp.ebi.ac.uk/pub/databases/gencode/Gencode_human/release_33/GRCh38.primary_assembly.genome.fa.gz # transcriptome wget ftp://ftp.ebi.ac.uk/pub/databases/gencode/Gencode_human/release_33/gencode.v33.transcripts.fa.gz # annotation wget ftp://ftp.ebi.ac.uk/pub/databases/gencode/Gencode_human/release_33/gencode.v33.annotation.gtf.gz
The files you would need are:
- FASTA file for the genome primary assembly
- FASTA file corresponding to the CDS regions / transcripts
- GTF file for the annotation
# genome wget ftp://ftp.ensembl.org/pub/release-99/fasta/homo_sapiens/dna/Homo_sapiens.GRCh38.dna_rm.primary_assembly.fa.gz # transcriptome wget ftp://ftp.ensembl.org/pub/release-99/fasta/homo_sapiens/cds/Homo_sapiens.GRCh38.cds.all.fa.gz # annotation wget ftp://ftp.ensembl.org/pub/release-99/gtf/homo_sapiens/Homo_sapiens.GRCh38.99.chr.gtf.gz
Our data set
To speed up the mapping process, we retrieved a subset of the FASTA and GTF files that correspond only to chromosome 6.
You can download them from:
# go to the appropriate folder cd ~/rnaseq_course/reference_genome # download reference files for chromosome 6 wget https://public-docs.crg.es/biocore/projects/training/PHINDaccess2020/reference_chr6_Hsapiens.tar.gz # extract archive tar -xvzf reference_chr6_Hsapiens.tar.gz # remove remaining .tar.gz archive rm reference_chr6_Hsapiens.tar.gz
The genome is often stored as a FASTA file (.fa file): each header (that can be chromosomes, transcripts, proteins), starts with “>”:
zcat reference_chr6/Homo_sapiens.GRCh38.dna.chrom6.fa.gz | head -n 1
The size of the chromosome (in bp) is already reported in the header, but we can check it as follows:
zcat ~/rnaseq_course/reference_genome/reference_chr6/Homo_sapiens.GRCh38.dna.chrom6.fa.gz | grep -v ">" | tr -d '\n' | wc -m # 170805979
The annotation is stored in General Transfer Format (GTF) format (which is an extension of the older GFF format): a tabular format with one line per genome feature, each one containing 9 columns of data. In general it has a header indicated by the first character ”#” and one row per feature composed in 9 columns:
|Column number||Column name||Details|
|1||seqname||name of the chromosome or scaffold; chromosome names can be given with or without the ‘chr’ prefix.|
|2||source||name of the program that generated this feature, or the data source (database or project name)|
|3||feature||feature type name, e.g. Gene, Variation, Similarity|
|4||start||Start position of the feature, with sequence numbering starting at 1.|
|5||end||End position of the feature, with sequence numbering starting at 1.|
|6||score||A floating point value.|
|7||strand||defined as + (forward) or - (reverse).|
|8||frame||One of ‘0’, ‘1’ or ‘2’. ‘0’ indicates that the first base of the feature is the first base of a codon, ‘1’ that the second base is the first base of a codon, and so on..|
|9||attribute||A semicolon-separated list of tag-value pairs, providing additional information about each feature.|
zcat reference_chr6/Homo_sapiens.GRCh38.88.chr6.gtf.gz | head -n 10
Let’s check the 9th field:
zcat reference_chr6/Homo_sapiens.GRCh38.88.chr6.gtf.gz | cut -f9 | head
Let’s check how many genes are in the annotation file:
zcat reference_chr6/Homo_sapiens.GRCh38.88.chr6.gtf.gz | grep -v "#" | awk '$3=="gene"' | wc -l # 2860
And get a final counts of every feature:
zcat reference_chr6/Homo_sapiens.GRCh38.88.chr6.gtf.gz | grep -v "#" | cut -f3 | sort | uniq -c